Determining the potentiative state of a chromatin domain.
نویسندگان
چکیده
amounts of CsCl by measuring the refractive index. The refractive index of the DNA solution was 1.3354, which is comparable to that obtained using a microconcentrator (3). Removal of CsCl is essential in obtaining purified plasmid DNA. A common method used to remove CsCl is dialysis against large volumes of TE buffer, which is more costly and timeconsuming than the method we describe. Alternatively, spin dialysis through a microconcentrator removes CsCl quickly, but at the cost of the microconcentrator. Finally, we have shown that using two ethanol precipitations is both inexpensive and fast. Therefore, this simple centrifugation step can at least double the yield of plasmid DNA when using ethanol precipitation to remove CsCl during largescale preparations of plasmid DNA.
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عنوان ژورنال:
- BioTechniques
دوره 22 5 شماره
صفحات -
تاریخ انتشار 1997